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Biowest瑗跨彮鐗欑搳鑴傜硸Agarose 100g 111860 鐢㈠搧鎸囨瑙ｈ畝
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瑗跨彮鐗欑搳鑴傜硸(鐝捐波淇冮姺锛夛細鏈€娆婂▉鐨勮タ鐝墮鐡婅剛绯栵紙Biowest Agarose锛夋槸涓€绋浕娉崇礆鍒ョ磾搴﹀緢楂樼殑鐢㈠搧锛屼綔鐐鸿憲鍚嶇殑鐡婅剛绯栧搧鐗岋紝鍏锋湁妤典匠鐨勭敘鍝佹€у児姣旓紝鍦ㄥ悓琛屼腑鏈夎憲闈炲父楂樼殑鐭ュ悕搴︼紝瑗跨彮鐗欑搳鑴傜硸锛圔iowest Agarose锛変笉鍍呴仼鐢ㄤ簬铔嬬櫧闆绘吵涔熷彲浠ョ敤浜庡叾瀹冩牳閰稿垎鏋愰浕娉炽€�
| 瑕忔牸 | 100g | |
| 鐢㈠湴 | 瑗跨彮鐗� | |
| 鍙冩暩锛圧egular锛� | EEO(electroendosmosis)(-Mr) | 0.15 |
| Gel Strength(1%)(g/cm2) | ≥750 | |
| Gelling temperature(1%)(鈩�) | 37±1.5 | |
| 瑾槑 | 鐢ㄤ簬鏃ュ父鏍搁吀鍒嗘瀽闆绘吵銆佽泲鐧介浕娉崇瓑 | |
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鏈€娆婂▉鐨勮タ鐝墮鐡婅剛绯栵紙Biowest Agarose锛夋槸涓€绋浕娉崇礆鍒ョ磾搴﹀緢楂樼殑鐢㈠搧锛屼綔鐐鸿憲鍚嶇殑鐡婅剛绯栧搧鐗岋紝鍏锋湁妤典匠鐨勭敘鍝佹€у児姣旓紝鍦ㄥ悓琛屼腑鏈夎憲闈炲父楂樼殑鐭ュ悕搴︼紝鍦ㄥ悓椤炵敘鍝佷腑鍗犳摎钁楁渶楂樼殑甯傚牬浠介锛岃タ鐝墮鐡婅剛绯栵紙Biowest Agarose锛変笉鍍呴仼鐢ㄤ簬铔嬬櫧闆绘吵涔熷彲浠ョ敤浜庡叾瀹冩牳閰稿垎鏋愰浕娉炽€� | |
鎶€琛撳弮鏁革細Agarose Low EEO
Gel Strength (1.0%) (g/cm2)………………≥750
Gelling Range (1.5%) (鈩�)………………36 - 39
Melting Range (鈩�)………………………87 - 89
EEO (-mr)……………………………………≤0.15
Sulfate (%)………………………………≤0.15
DNase……………………………………None Detected
RNase……………………………………None Detected
Protease………………………………None Detected
Introduction:
Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID).
Analysis Note :
Sulfate content- used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength- the force that must be applied to a gel to cause it to fracture.
Gel point- the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO)- a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Gel strength- the force that must be applied to a gel to cause it to fracture.
Gel point- the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO)- a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Preparation of Agarose Gels for DNA separations:
Weigh out the desired amount of agarose and place in an Erlenmeyer flask with a measured amount of electrophoresis buffer, e.g. for an 0.8% gel, add 0.8 gm of agarose and 100ml of TBE Buffer (1X), to a 200 ml flask. The larger flask insures against the agarose boiling over. Dissolve the agarose in a boiling water bath or in a revolving-plate microwave oven. All the grains of agarose should be dissolved and the solution clear. Cool the solution to 60°C ( 70°C for concentrations 2% or above) and pour immediately. Allow the gel to set for one-half hour before using. Make sure to use the same electrophoresis buffer in the gel as for the running buffer.
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